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COA checks & dose maths

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Reconstitution and dose maths

MOTS-c recon­stitution calculator

A 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL, so a 2.5 mg dose is 0.5 mL: 50 units on a U-100 insulin syringe. The 10 mg vial is nearly the only MOTS-c size in circulation, so this page's defaults will probably match the vial in your hand.

Updated 30 August 2026. Ledger figures captured 30 August 2026.

The arithmetic, shown

  1. 10 mg in the vial ÷ 2 mL of water = 5.00 mg/mL
  2. 2.500 mg per dose ÷ 5.00 mg/mL = 0.500 mL
  3. 0.500 mL × 100 = 50 units on a U-100 syringe
MOTS-c ยท 10 mg vial U-100
Insulin syringe showing the units to draw
Draw to 50 units
Volume 0.500 mL
Doses / vial 4
2.50 mg

Your vial

Vial size (mg)

Bacteriostatic water added (mL)

Resulting concentration 5.00 mg/mL
Check a report for this vial

What the calculator is doing

Milligrams in the vial, units on the barrel.

A 10 mg peptide vial plus 2 mL of bacteriostatic water gives a 5 mg/mL solution, and a 500 microgram dose is drawn to the 10 unit mark on a U-100 syringe.
A worked example, not your numbers: a 10 mg vial with 2 mL of bacteriostatic water gives 5 mg/mL. A 500 mcg dose is 0.1 mL of that solution, which is the 10 unit mark on a U-100 syringe. The dose is in micrograms and the draw is in units, and they are never the same number.

Common draws

The usual vials, worked out.

MOTS-c is dosed in milligrams from 5 and 10 mg vials, and the doses are large relative to the vial: a 10 mg vial at 5 mg per dose holds just two. Watch the top of the syringe here; at the wrong concentration a full dose lands at or past 100 units.

MOTS-c draws at common vial and water combinations, U-100 syringe
VialWater addedConcentrationDoseDraw toDoses in the vial
5 mg1 mL5.00 mg/mL2.5 mg50 units2
5 mg2 mL2.50 mg/mL1 mg40 units5
10 mg1 mL10.00 mg/mL2.5 mg25 units4
10 mg2 mL5.00 mg/mL2.5 mg50 units4
10 mg1 mL10.00 mg/mL5 mg50 units2
10 mg3 mL3.33 mg/mL2.5 mg75 units4

Each row is the calculator's three steps in print: vial ÷ water = mg/mL, dose ÷ mg/mL = volume, volume × 100 = units on a U-100 syringe. Doses in the vial is the labelled mass divided by the dose, rounded down. Note what is missing: 5 mg from a 2 mL reconstitution of a 10 mg vial is exactly 100 units, a completely full syringe.

Label against assay

A "10 mg" vial that held 11.32 mg.

Every number this calculator produces starts from the label you select, and the label is a claim. The public record happens to hold a MOTS-c example of the gap: Janoshik report 216154, a vial sold as MOTS-c 10 mg, assayed at 11.32 mg with 98.872% purity. Same product, 13% more substance than the label said.

An overfilled vial is the benign direction, and it still moves every dose drawn from it by the same 13%. The arithmetic on this page cannot see that; only an assay can. That is the working relationship between the two tools on this site: the calculator turns a label into a draw, and report verification tells you whether the label deserved the trust.

The public record

One vial size, over and over.

15

of the 201 tests in Janoshik's public archive name MOTS-c in the listing, 14 of them as a 10 mg vial. Captured 30 August 2026.

Fourteen of fifteen listings claim the same 10 mg size, several submitted in a single batch by one manufacturer. No other compound in the archive is this uniform, which is why this page defaults to the 10 mg vial rather than making you choose.

Uniform sizing is convenience, not assurance: the listings are wholesale-side, and a public report proves one batch was tested once. If your vial came with a report, verify it against the lab, and the report ledger explains what a listing proves and what it does not.

Questions

MOTS-c dose maths, asked directly.

How many units is 2.5 mg of MOTS-c?+

At 5 mg/mL, which is a 10 mg vial reconstituted with 2 mL of bacteriostatic water, 2.5 mg is 0.5 mL: 50 units on a U-100 insulin syringe. At 10 mg/mL, the same vial with 1 mL, it is 25 units.

How many units is 5 mg of MOTS-c?+

At 5 mg/mL, exactly 100 units, which is a completely full 1 mL U-100 syringe. Either reconstitute with less water (1 mL gives 10 mg/mL and a 50-unit draw) or split the dose across two draws. A full-to-the-line syringe leaves no room to correct an air bubble.

How many doses are in a 10 mg vial of MOTS-c?+

At 2.5 mg per dose, 4 doses. At 5 mg, 2. Divide the labelled vial mass by the dose and round down; the calculator shows this in the doses-per-vial readout.

Does a 10 mg label mean the vial holds 10 mg?+

Not necessarily, and there is a public example. Janoshik report 216154, a MOTS-c vial labelled 10 mg, assayed at 11.32 mg with 98.872% purity. This calculator computes from the label you select; actual content is a testing question, which is what a lab report answers.

Legal status

Research use only

MechPeptides sells nothing. MOTS-c is named on this page so the arithmetic has a label on it. It is not approved by the FDA for human or veterinary use, it is not a dietary supplement, and nothing on this site is medical advice or a recommended protocol.

The calculator performs arithmetic on numbers you enter. It converts a vial mass and a diluent volume into a concentration, then into a volume and a U-100 syringe reading. It does not know your compound, it does not recommend a dose, and its slider bounds are display ranges rather than guidance. Confirm every calculation independently before acting on it.